This database of renal inner medullary collecting duct (IMCD) phosphoproteins is based on protein mass spectrometry data from the NHLBI Laboratory of Kidney and Electrolyte Metabolism. All data are from IMCD cells freshly purified from rat inner medullas.
Mass spectrometry (MS) used and data filtering:
FT: Spectral data were acquired using the FT-ICR analyzer for full MS (parent ion) scans. All FT spectra were manually confirmed.
LTQ: Spectral data were acquired using the LTQ analyzer for full MS (parent ion) scans. LTQ spectra were filtered for a false positive rate < 1% after database searching using the target-decoy approach. Some LTQ spectra were manually confirmed (Expt. 3).
1) IMCD samples were treated with 100 nM calyculin A (a Ser/Thr phosphatase inhibitor) for 30 min.
2) IMCD samples were treated with 0.1 nM dDAVP (a V2-receptor-specific vasopressin analog) for 10 min.
3) IMCD membrane samples +/- short-term vasopressin.
4) IMCD samples were treated with 0.1 nM dDAVP and sonicated in 8M urea.
Hoffert et al., Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A. 2006 May 2;103(18):7159-64.
Hoffert et al., An Automated Platform for Analysis of Phosphoproteomic Datasets: Application to Kidney Collecting Duct Phosphoproteins. J Proteome Res. 2007 Sep;6(9):3501-8.
The CDPD was created by Jason Hoffert, Trairak Pisitkun, and Mark Knepper. Contact us with questions or comments at firstname.lastname@example.org.
Current Database Size: 367 unique proteins. ^ indicates an ambiguous phosphorylation site. Database updated: Januaray, 22, 2009 (download data)
To sort the database by Protein Name, Function, or Family/Domain, click the header of each columns.