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Department of Health and Human ServicesNational Institutes of HealthNational Heart Lung and Blood Institute
mpkCCD Cell Phosphoproteomic Database (mCPD)
NHLBI Division of Intramural ResearchLaboratory of Kidney and Electrolyte Metabolism

The mpkCCD Cell Phosphoproteomic Database (mCPD) contains protein mass spectrometry data from the NHLBI Laboratory of Kidney and Electrolyte Metabolism. All data are obtained from a clonal mpkCCD cell line "clone 11" (Yu et al.1),which is derived from mouse cortical collecting duct (Duong van Hoyen et al. 2).

Experiment description:
The cells were grown on membrane supports until confluence and treated with the vasopessin V2 receptor specific agonist dDAVP for 3 days (1nM). After withdrawal of the hormone for 6h, cells were treated with dDAVP 0.1nM or vehicle for 30 min. All experiments were conducted using Stable Isotope Labeling by Amino-Acids in Cell culture (SILAC), which enables quantification of the hormone effect for a large number of peptides. If available, the ratio log2(dDAVP/vehicle) is given for the corresponding peptide. For more details, see the method section of the current paper (the reference will be added here upon publication of the manuscript).

The mCPD was created by Markus M. Rinschen, Ming-Jiun Yu, Guozhong Ma, Trairak Pisitkun, and Mark A. Knepper. Contact us with questions or comments at knepperm@nhlbi.nih.gov.

References:
  1. Yu, M. J., Miller, R. L., Uawithya, P., Rinschen, M. M., Khositseth, S., Braucht, D. W., Chou, C. L., Pisitkun, T., Nelson, R. D. & Knepper, M. A. Proc. Natl. Acad. Sci. U. S. A 106, 2441-2446, 2009
  2. Duong Van Huyen JP, Bens M and Vandewalle A. Differential Effects of Aldosterone and Vasopressin on Chloride Fluxes in Transimmortalized Mouse Cortical Collecting Duct Cells. Journal of Membrane Biology 164: 79-90, 1998