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This Kidney and Epididymal V-ATPase-rich Cell Proteome Database
is based on protein mass spectrometry data from the NHLBI Laboratory of
Kidney and Electrolyte Metabolism. Specialized proton-transporting cells
are located in several tissues including the kidney and epididymis where
they actively acidify the extracellular environment. These cells, which
include renal intercalated cells and epididymal clear cells, express high
levels of the V-ATPase B1 subunit in their plasma membrane. We provide
here a comprehensive catalog of the proteins that are expressed in these
cells based on their isolation by enzymatic digestion from tissues and
fluorescence-activated cell sorting (FACS) from transgenic B1-EGFP mice,
which express EGFP under the control of the promoter of the V-ATPase-B1
subunit. In these mice, type A and B intercalated cells and connecting
segment principal cells of the kidney, and narrow and clear cells of the
epididymis express EGFP, while all other cell types are negative.
The proteomic signatures of kidney and epididymal EGFP+ cells were
identified by LC-MS/MS and compared to their respective EGFP- cell
populations.
Reference:
Kidney V-ATPase-rich Cell Proteome Database - 2297 proteins:
Epididymal V-ATPase-rich Cell Proteome Database - 1564 proteins:
The Kidney and Epididymal V-ATPase-rich Cell Proteome Database was created by Trairak Pisitkun, Nicolas Da Silva, Dennis Brown, Sylvie Breton, and Mark A. Knepper.
For questions or comments please contact Sylvie Breton at Breton.Sylvie@mgh.harvard.edu or Mark A. Knepper at knep@helix.nih.gov.
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