Confocal Microscopy Technology
Enzyme Dependent-Fluorescence Recovery After Photobleaching (ED-FRAP)
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ED-FRAP is a method of quantitatively imaging the distribution of enzymatic activity within small samples or cells.
The method relies on the selective destruction of a metabolite using a photobleaching pulse and measuring the rate of enzymatically driven recovery of the metabolite.
NADH ED-FRAP is dependent on the presence of enzyme in vitro
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Before |
Bleach |
Recover |
ED-FRAP is Linearly Enzyme Dependant In Vitro
ED-FRAP and Intact Cardiac Myocytes
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Before |
Bleach |
Recover |
Regional ED-FRAP in Intact Cardiac Myocytes
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Control NADH Fluorescence Image |
ED-FRAP NADH Recovery Rate Constant Image |
The rate constant map is basically an image of NADH generating Capacity in the cell. Note the prevalence of mitochondrial in this process.
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