LDB Mouse ENU Mutagenesis Program
The Laboratory of Developmental Biology initiated on June 2002,
a mouse ENU Mutagenesis Program. The goal of this program is to
use noninvasive ultrasound imaging to conduct a noninvasive in utero
screen of ENU mutagenized mice to recover mouse mutations causing
congenital heart anomalies. As of August 15, 2003, 3869 fetuses
have been screened from 529 G2 females encompassing nearly 200 families,
yielding a high incidence of cardiovascular anomalies (see Summary
of Mutagenesis Screening). This work is being conducted in collaboration
with the Jackson Laboratory, under the auspices of a guest
investigator program funded by the NHLBI Program for Genomic
Applications.
For this screen, C57BL6/J mice are ENU mutagenized and bred using
a backcrossing scheme designed for the retrieval of recessive mutations.
Non-invasive in utero fetal ultrasound imaging was used for this
study and includes 2D imaging, spectral Doppler analysis, color
flow Doppler, and M-mode imaging. Fetuses are scanned from E12.5
to E19.5 (birth), with most scanned horizontally at two or three
different developmental stages. Overall, the gross defects that
can be detected by ultrasound include prenatal lethality, growth
retardation, and cardiovascular defects. Cardiovascular defects
that can be ultrasound diagnosed including arrhythmia, heart failure,
high outflow velocity, outflow regurgitation, hypertrophy and ectopia
cordis.
Links:
NHLBI LDB Photo Gallery
Web Site
Mouse Images Collection
System (MICS)
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