| In Vitro Motility Assay
Schematic of flowcell
Description of schematic of the flow cell used
in the invitro motility assay.
On top of a microsocpe slide, 2 spacers are placed on which
a coverslip is then placed. This creates the flow cell for
samples. Samples are added at one end, migrating through the
flow cell chamber, sticking to the coverslip.
A magnified View
Description of a magnified view from the motility assay:
Coverslip surface is coated first with BSA, then myosin is
bound to that the same surface, and then fluorescently labled
actin binds to the myosin.
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