Connexin Subtype Distribution
Demonstration of Cx45 knockout mouse embryos (E9.5) with LacZ expression
driven by the Cx45 promoter in place of endogenous Cx45 expression.
A) Wild-type B-E) Heterozygous Cx45 knockout, F) Homozygous Cx45
knockout.
NB: Apparent gene dosing of LacZ expression, with heterozygous
Cx45 knockout embryos displaying lighter stain than the homozygous
embryo. Also note that LacZ expression appears stronger in the outflow
region of the heart than the presumptive left ventricle
Scale bars represent 250 mm. (Image provided by Richard Francis)
Views of the dissected Heart tube from Cx45 knockout mouse embryos
(E9.5) with LacZ expression driven by the Cx45 promoter in place
of endogenous Cx45 expression.
Figure 1 demonstrates heart tube and attached thoracic segment
from wild type (1A), Heterozygous Cx45 knockout (1B 1C), and Homozygous
Cx45 knockout (1D) embryos.
Figure 2 Magnified views of heart tubes shown in figure 1. 2A,
2C front and rear view of the heart tube shown in 1C (heterozygous
Cx45 knockout). 2B, 2D front and rear view of the heart tube shown
in 1D (homozygous Cx45 knockout).
NB: Stronger LacZ expression in outflow tract and the heart tube
segment connecting the presumptive left ventricle and the presumptive
left atrium.
Scale bars represent 250 mm. (Image provided by Richard Francis)
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