<% function getFileName() Dim pos, path, name path = Request.ServerVariables("PATH_INFO") pos = InStrRev(path,"/",-1,1) name = Right(path, len(path) - pos) 'response.write("PATH_TRANSLATED" + Request.ServerVariables("PATH_TRANSLATED") + "
") '**** Test Code *********************** getFileName = name 'Response.Write("filename for NS6 " + name + "
") 'Response.Write(strMainPhysicalPath & Replace(Request.QueryString("NAME"),"%2E",".")) End Function ' Function IsEmailValid(strEmail) ' Action: checks if an email is correct. ' Parameter: strEmail - the Email address ' Returned value: on success it returns True, else False. Function IsEmailValid(strEmail) Dim strArray Dim strItem Dim i Dim c Dim blnIsItValid ' assume the email address is correct blnIsItValid = True ' split the email address in two parts: name@domain.ext strArray = Split(strEmail, "@") ' if there are more or less than two parts If UBound(strArray) <> 1 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check each part For Each strItem In strArray ' no part can be void If Len(strItem) <= 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check each character of the part ' only following "abcdefghijklmnopqrstuvwxyz_-." ' characters and the ten digits are allowed For i = 1 To Len(strItem) c = LCase(Mid(strItem, i, 1)) ' if there is an illegal character in the part If InStr("abcdefghijklmnopqrstuvwxyz_-.", c) <= 0 And Not IsNumeric(c) Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If Next ' the first and the last character in the part cannot be . (dot) If Left(strItem, 1) = "." Or Right(strItem, 1) = "." Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If Next ' the second part (domain.ext) must contain a . (dot) If InStr(strArray(1), ".") <= 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check the length oh the extension i = Len(strArray(1)) - InStrRev(strArray(1), ".") ' the length of the extension can be only 2, 3, or 4 ' to cover the new "info" extension If i <> 2 And i <> 3 And i <> 4 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' after . (dot) cannot follow a . (dot) If InStr(strEmail, "..") > 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' finally it's OK IsEmailValid = blnIsItValid End Function %> <% if LCase(getFilename)= "inthelab.asp" then response.write("Work being done in the Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI") end if if LCase(getFilename) = "labmembers.asp" then response.write("Staff, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI") end if if LCase(getFilename) = "publications.asp" then response.write("Publications, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "contactus.asp" then response.write("Geller Lab Contact, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "location.asp" then response.write("Nabel Lab Location") end if if LCase(getFilename) = "imagegallery.asp" then response.write("Geller Lab Images, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "favoritejournals.asp" then response.write("Favorite Journals, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "macroanalysis.asp" then response.write("Macro Analysis, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "labmethods.asp" then response.write("Lab Methods, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if %> - NIH <% Dim image_intheLab Dim image_labmembers Dim image_publications Dim image_contactus Dim image_location Dim image_imageGallery Dim image_nabelLabHOme Dim image_favoriteJournals Dim image_labMethods image_intheLab = "arrow_off.gif" image_labmembers = "arrow_off.gif" image_publications = "arrow_off.gif" image_contactus = "arrow_off.gif" image_location = "arrow_off.gif" image_imageGallery = "arrow_off.gif" image_nabelLabHome = "arrow_off.gif" image_favoriteJournals = "arrow_off.gif" image_labMethods = "arrow_off.gif" if LCase(getFilename)= "inthelab.asp" or LCase(getFilename)= "indexns6.asp" then image_intheLab="arrow_on.gif" end if if LCase(getFilename) = "labmembers.asp" then image_labmembers = "arrow_on.gif" end if if LCase(getFilename) = "publications.asp" then image_publications = "arrow_on.gif" end if if LCase(getFilename) = "contactus.asp" then image_contactus = "arrow_on.gif" end if if LCase(getFilename) = "location.asp" then image_location = "arrow_on.gif" end if if LCase(getFilename) = "imagegallery.asp" then image_imageGallery = "arrow_on.gif" end if if LCase(getFilename) = "nabelLabeHome.asp" then image_nabelLabeHome = "arrow_on.gif" end if if LCase(getFilename) = "favoritejournals.asp" then image_favoriteJournals = "arrow_on.gif" end if if LCase(getFilename) = "labmethods.asp" then image_labMethods = "arrow_on.gif" end if %>
Geller Lab Home Jump pass to content National Heart, Lung, and Blood Institute
Developmental Neurobiology 		Jump pass to links
[Border] <%if image_inthelab="arrow_on.gif" then%> In the Lab <%end if%> <%if image_labmembers="arrow_on.gif" then%> Lab Members <%end if%> <%if image_publications="arrow_on.gif" then%> Publications <%end if%> <%if image_contactus="arrow_on.gif" then%> Contact Us <%end if%> <%if image_location="arrow_on.gif" then%> Location <%end if%> <%if image_imageGallery="arrow_on.gif" then%> Image Gallery <%end if%> <%if image_nabelLabHome="arrow_on.gif" then%> Geller Lab Home <%end if%> <%if image_favoriteJournals="arrow_on.gif" then%> Favorite Journals <%end if%> <%if image_labMethods="arrow_on.gif" then%> Lab Methods <%end if%>  
Table image of part of Helix NHLBI Home NHLBI Labs    
arrow image to point to Nabel Lab splash page<%if image_nabelLabHome="arrow_off.gif" then %><%end if%>[Nabel Lab Home]<%if image_nabelLabHome="arrow_off.gif" then %><%end if%>arrow image to point to in the lab page<%if image_intheLab="arrow_off.gif" then %><%end if%>[In the Lab]<%if image_intheLab="arrow_off.gif" then%><%end if%>arrow image to point to in the lab members page<%if image_labmembers="arrow_off.gif" then %><%end if%>[Lab Members]<%if image_labmembers="arrow_off.gif" then %><%end if%>arrow image to point to publications page<%if image_publications="arrow_off.gif" then %><%end if%>[Publications]<%if image_publications="arrow_off.gif" then %><%end if%>arrow image to point to Contact page<%if image_contactus="arrow_off.gif" then %><%end if%>[Contact Us]<%if image_contactus="arrow_off.gif" then %><%end if%>arrow image to point to lab location page<%if image_location="arrow_off.gif" then %><%end if%>[Location]<%if image_location="arrow_off.gif" then %><%end if%>arrow image to point to Favorite Journals<%if image_favoriteJournals="arrow_off.gif" then %><%end if%>[Favorite Journals]<%if image_favoriteJournals="arrow_off.gif" then %><%end if%> arrow image to point to Lab Methods<%if image_labMethods="arrow_off.gif" then %><%end if%>[Lab Methods]<%if image_labMethods="arrow_off.gif" then %><%end if%>

 

Reagents / Solutions

  • Cells! (at least 5 x 106).
  • Fetal calf serum (FCS), heat inactivated at 65 °C for 1 hr.
  • Dimethylsulfoxide (DMSO).
  • Freezing vials - labeled with cell type, date and your initials.
  • Plastic or cardboard box for initial freezing of cells
  • Canes for liquid nitrogen tank
  • Liquid nitrogen tank (in cold room).

Protocol (6/1/00)

  1. Spin down cells, remove and discard the supernatant.
  2. Resuspend the cells at 5 x 106 per ml in FCS.
  3. Add DMSO to 10% (by volume) dropwise, agitating gently after each drop. Do this slowly.
  4. Place 1ml aliquots into the vials.
  5. Put vials into a rack inside a box.
  6. Place lid on box and seal in place with tape.
  7. Place box in the -80º freezer. Leave for 6 - 24 hrs to freeze.
  8. Remove vials from box and place in canes and lower into liquid nitrogen.  Be careful not to get splattered!
  9. Test the efficiency of the freezing procedure by thawing one vial (method).
  10. If the cells were healthy (> 95% viable) at the start of this procedure, then cells should be 80-90% viable 24 hours after thawing.
Table image of part of HelixTable image of Helix

 

Dr. Herbert Geller, Head
Developmental Neurobiology Section, NHLBI, NIH
Building 10, Room 6D18, 10 Center Drive, MSC-1754
Bethesda, MD 20892-1650
Email: gellerh@nhlbi.nih.gov
NHLBI HomeLabs at the NHLBIContact the NHLBIAbout NHLBISearch NHLBI
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