<% function getFileName() Dim pos, path, name path = Request.ServerVariables("PATH_INFO") pos = InStrRev(path,"/",-1,1) name = Right(path, len(path) - pos) 'response.write("PATH_TRANSLATED" + Request.ServerVariables("PATH_TRANSLATED") + "
") '**** Test Code *********************** getFileName = name 'Response.Write("filename for NS6 " + name + "
") 'Response.Write(strMainPhysicalPath & Replace(Request.QueryString("NAME"),"%2E",".")) End Function ' Function IsEmailValid(strEmail) ' Action: checks if an email is correct. ' Parameter: strEmail - the Email address ' Returned value: on success it returns True, else False. Function IsEmailValid(strEmail) Dim strArray Dim strItem Dim i Dim c Dim blnIsItValid ' assume the email address is correct blnIsItValid = True ' split the email address in two parts: name@domain.ext strArray = Split(strEmail, "@") ' if there are more or less than two parts If UBound(strArray) <> 1 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check each part For Each strItem In strArray ' no part can be void If Len(strItem) <= 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check each character of the part ' only following "abcdefghijklmnopqrstuvwxyz_-." ' characters and the ten digits are allowed For i = 1 To Len(strItem) c = LCase(Mid(strItem, i, 1)) ' if there is an illegal character in the part If InStr("abcdefghijklmnopqrstuvwxyz_-.", c) <= 0 And Not IsNumeric(c) Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If Next ' the first and the last character in the part cannot be . (dot) If Left(strItem, 1) = "." Or Right(strItem, 1) = "." Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If Next ' the second part (domain.ext) must contain a . (dot) If InStr(strArray(1), ".") <= 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check the length oh the extension i = Len(strArray(1)) - InStrRev(strArray(1), ".") ' the length of the extension can be only 2, 3, or 4 ' to cover the new "info" extension If i <> 2 And i <> 3 And i <> 4 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' after . (dot) cannot follow a . (dot) If InStr(strEmail, "..") > 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' finally it's OK IsEmailValid = blnIsItValid End Function %> <% if LCase(getFilename)= "inthelab.asp" then response.write("Work being done in the Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI") end if if LCase(getFilename) = "labmembers.asp" then response.write("Staff, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI") end if if LCase(getFilename) = "publications.asp" then response.write("Publications, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "contactus.asp" then response.write("Geller Lab Contact, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "location.asp" then response.write("Nabel Lab Location") end if if LCase(getFilename) = "imagegallery.asp" then response.write("Geller Lab Images, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "favoritejournals.asp" then response.write("Favorite Journals, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "boundmov.asp" then response.write("Growth Cones Turn at a Boundary, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if if LCase(getFilename) = "speckles.asp" then response.write("The End-Binding Protein EB3 Localizes Points of Microtubule Polymerization in a Neuron, Developmental Neurobiology Lab, Div. of Intramural Research, NHLBI ") end if %> - NIH <% Dim image_intheLab Dim image_labmembers Dim image_publications Dim image_contactus Dim image_location Dim image_imageGallery Dim image_nabelLabHOme Dim image_favoriteJournals Dim image_labMethods image_intheLab = "arrow_off.gif" image_labmembers = "arrow_off.gif" image_publications = "arrow_off.gif" image_contactus = "arrow_off.gif" image_location = "arrow_off.gif" image_imageGallery = "arrow_off.gif" image_nabelLabHome = "arrow_off.gif" image_favoriteJournals = "arrow_off.gif" image_labMethods = "arrow_off.gif" if LCase(getFilename)= "inthelab.asp" or LCase(getFilename)= "indexns6.asp" then image_intheLab="arrow_on.gif" end if if LCase(getFilename) = "labmembers.asp" then image_labmembers = "arrow_on.gif" end if if LCase(getFilename) = "publications.asp" then image_publications = "arrow_on.gif" end if if LCase(getFilename) = "contactus.asp" then image_contactus = "arrow_on.gif" end if if LCase(getFilename) = "location.asp" then image_location = "arrow_on.gif" end if if LCase(getFilename) = "imagegallery.asp" then image_imageGallery = "arrow_on.gif" end if if LCase(getFilename) = "nabelLabeHome.asp" then image_nabelLabeHome = "arrow_on.gif" end if if LCase(getFilename) = "favoritejournals.asp" then image_favoriteJournals = "arrow_on.gif" end if if LCase(getFilename) = "labmethods.asp" then image_labMethods = "arrow_on.gif" end if %>
Geller Lab Home Jump pass to content National Heart, Lung, and Blood Institute
Developmental Neurobiology
Jump pass to links
[Border] <%if image_inthelab="arrow_on.gif" then%> In the Lab <%end if%> <%if image_labmembers="arrow_on.gif" then%> Lab Members <%end if%> <%if image_publications="arrow_on.gif" then%> Publications <%end if%> <%if image_contactus="arrow_on.gif" then%> Contact Us <%end if%> <%if image_location="arrow_on.gif" then%> Location <%end if%> <%if image_imageGallery="arrow_on.gif" then%> Image Gallery <%end if%> <%if image_nabelLabHome="arrow_on.gif" then%> Geller Lab Home <%end if%> <%if image_favoriteJournals="arrow_on.gif" then%> Favorite Journals <%end if%> <%if image_labMethods="arrow_on.gif" then%> Lab Methods <%end if%>  
Table image of part of Helix NHLBI Home NHLBI Labs    
arrow image to point to Nabel Lab splash page<%if image_nabelLabHome="arrow_off.gif" then %><%end if%>[Nabel Lab Home]<%if image_nabelLabHome="arrow_off.gif" then %><%end if%>arrow image to point to in the lab page<%if image_intheLab="arrow_off.gif" then %><%end if%>[In the Lab]<%if image_intheLab="arrow_off.gif" then%><%end if%>arrow image to point to in the lab members page<%if image_labmembers="arrow_off.gif" then %><%end if%>[Lab Members]<%if image_labmembers="arrow_off.gif" then %><%end if%>arrow image to point to publications page<%if image_publications="arrow_off.gif" then %><%end if%>[Publications]<%if image_publications="arrow_off.gif" then %><%end if%>arrow image to point to Contact page<%if image_contactus="arrow_off.gif" then %><%end if%>[Contact Us]<%if image_contactus="arrow_off.gif" then %><%end if%>arrow image to point to lab location page<%if image_location="arrow_off.gif" then %><%end if%>[Location]<%if image_location="arrow_off.gif" then %><%end if%>arrow image to point to Favorite Journals<%if image_favoriteJournals="arrow_off.gif" then %><%end if%>[Favorite Journals]<%if image_favoriteJournals="arrow_off.gif" then %><%end if%> arrow image to point to Lab Methods<%if image_labMethods="arrow_off.gif" then %><%end if%>[Lab Methods]<%if image_labMethods="arrow_off.gif" then %><%end if%>

 

The End-Binding Protein EB3 Localizes Points of Microtubule Polymerization in a Neuron

Below is a link to a Quick Time Movie of a cultured neonatal mouse cerebellar granule neuron transfected with a construct of monomeric RFP-EB3.    A round glass coverslip which formed the bottom of a 35 mm Petri dish was first coated with poly-l-lysine (PLL).  Eight day old mouse cerebellar granule neurons were dissociated, transfected with RFP-EB3 and plated on the coverslip.  After 24 hr, the Petri dish was placed in a CO2 incubator on the stage of a Nikon inverted microscope.  Images were captured every 5 sec with a 100X objective using the Metamorph Program.  The Quick Time movie was made using ImageJ and represents 25 frames.  What you will see is a neuron with EB3 speckles moving outward from the soma and distally in each of the processes. Speckles indicate the sites of tubulin polymerization and the rate of movement of speckles represents the polymerization rate. 

Download the Movie

Table image of part of HelixTable image of Helix

 

Dr. Herbert Geller, Head
Developmental Neurobiology, NHLBI, NIH
Building 10, Room 8C-213, 10 Center Drive, MSC-1650
Bethesda, MD 20892-1650
Email: gellerh@nhlbi.nih.gov

NHLBI HomeLabs at the NHLBIContact the NHLBIAbout NHLBISearch NHLBI
Table image of Helix

 

 

Accessibility Policy | Privacy Statement
Health Information | Scientific Resources | Research Funding
News and Press Releases | Committees, Meetings, Events | Clinical Guidelines
Studies Seeking Patients | Labs at the NHLBI | Technology Transfer


Logo DHHS
Logo NIH
Logo NHLBI

Partners logo Cardiovascular Health Partners
Healthy People 2010

Please send us your feedback, comments, and questions
by using the appropriate link on the page, Contact the NHLBI.

Note to users of screen readers and other assistive technologies: please report your problems here.