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Optical Spectroscopy Section

Structural Biophysics Section

Protein Conformation and Dynamics Research

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Protein Conformation and Dynamics Research

 

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Instrumentation

Time-resolved Spectroscopy

Regeneratively-amplified femtosecond Ti:sapphire laser
      775 nm, 1 mJ, 1kHz

Optical parametric amplifier
      280—2000 nm, 1—100 mJ

Nanosecond Ti:sapphire laser
800—900 nm, 1 mJ, single shot-4kHz

Harmonic generators (266-300;400-450nm)

Cuvette with excited fluorescent sample

Picosecond streak camera
      0.2—50 ns

Stopped-flow mixer
      0.25—5.0 ms dead time,
      4 independent syringes

Schematic of time resolved fluorescence decay collection: Laser excitation of sample, collection of florescence emission, and streak camera with CCD
Image of fluorescence decay collected with streak camera and subsequent computer readout
Laser Table with optical parametric amplifier and streak camera
Stopped-flow mixer with photomultiplier tube on small laser table
Laser lab with view of the fluorimeter

Steady-state Spectroscopy

UV-VIS Absorption
      Cary 300 Bio

Emission
      Horiba Jobin Yvon Fluorolog-3

CCD Spectrographs
      Ocean Optics USB4000: 200-850nm
      Ocean Optics HR2000+:
      0.04 nm resolution, full spectrum
      every 1ms, 310-750nm

CW Lasers
      DPSS: 447.8 nm, 3-40mW
      Helium Neon: 633nm, 20mW
Fume hood with Schlenk Line in wet lab

Wet Chemistry

Schlenk Line
   Anaerobic sample
   manipulation
   Sample deoxygenation

Gel Electrophoresis
   Pharmacia Biotech
   PhastSystem,
   SDS, Native, DNA

Chromatography
   FPLC ÃKTApurifier 10

Refrigerated chromatography system in wet lab

 
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