Genotyping Automations

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Click on the Staff Members name to access their Bio Page | : Link to Staff Members Email.

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<% rank = "50" %> <% if rank = 1 then %> <% end if%> <%if homepage = "webpage" then%> Richard Francis <%else%> Richard Francis <%end if%> <% if rank = 1 then %> <% end if%>
<% rank = "50" %> <% if rank = 1 then %> <% end if%> <%if homepage = "webpage" then%> Xin Xu <%else%> Xin Xu <%end if%> <% if rank = 1 then %> <% end if%>
<% rank = "50" %> <% if rank = 1 then %> <% end if%> <%if homepage = "webpage" then%> Ying-Jie Wu <%else%> Ying-Jie Wu <%end if%> <% if rank = 1 then %> <% end if%>

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Click on the Staff Members name to access their Bio Page | : Link to Staff Members Email.

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Kaari Linask <%else%>

Kaari Linask <%end if%> <% if rank = 1 then %> <% end if%>

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Click on the Staff Members name to access their Bio Page | : Link to Staff Members Email.

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<% rank = "50" %> <% if rank = 1 then %> <% end if%> <%if homepage = "webpage" then%> Heidi Dudik <%else%> Heidi Dudik <%end if%> <% if rank = 1 then %> <% end if%>
<% rank = "50" %> <% if rank = 1 then %> <% end if%> <%if homepage = "webpage" then%> Jay Noor <%else%> Jay Noor <%end if%> <% if rank = 1 then %> <% end if%>

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Click on the Staff Members name to access their Bio Page | : Link to Staff Members Email.

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Click on the Staff Members name to access their Bio Page | : Link to Staff Members Email.

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<% homepage = "" %> <% ' Function IsEmailValid(strEmail) ' Action: checks if an email is correct. ' Parameter: strEmail - the Email address ' Returned value: on success it returns True, else False. Function IsEmailValid(strEmail) Dim strArray Dim strItem Dim i Dim c Dim blnIsItValid ' assume the email address is correct blnIsItValid = True ' split the email address in two parts: name@domain.ext strArray = Split(strEmail, "@") ' if there are more or less than two parts If UBound(strArray) <> 1 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check each part For Each strItem In strArray ' no part can be void If Len(strItem) <= 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check each character of the part ' only following "abcdefghijklmnopqrstuvwxyz_-." ' characters and the ten digits are allowed For i = 1 To Len(strItem) c = LCase(Mid(strItem, i, 1)) ' if there is an illegal character in the part If InStr("abcdefghijklmnopqrstuvwxyz_-.", c) <= 0 And Not IsNumeric(c) Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If Next ' the first and the last character in the part cannot be . (dot) If Left(strItem, 1) = "." Or Right(strItem, 1) = "." Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If Next ' the second part (domain.ext) must contain a . (dot) If InStr(strArray(1), ".") <= 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' check the length oh the extension i = Len(strArray(1)) - InStrRev(strArray(1), ".") ' the length of the extension can be only 2, 3, or 4 ' to cover the new "info" extension If i <> 2 And i <> 3 And i <> 4 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' after . (dot) cannot follow a . (dot) If InStr(strEmail, "..") > 0 Then blnIsItValid = False IsEmailValid = blnIsItValid Exit Function End If ' finally it's OK IsEmailValid = blnIsItValid End Function %> <% end if %> <% if currentFolder = "emailForm" then%> <% function getEmail(name) if name = "Bishwanath Chatterjee" then getEmail = "ChatterB@nhlbi.nih.gov" end if if name = "Chih-Jen Wei" then getEmail = "WeiC@nhlbi.nih.gov" end if if name = "Diana Walker" then getEmail = "WalkerD@nhlbi.nih.gov" end if if name = "Qing Yu" then getEmail = "YuQ@nhlbi.nih.gov" end if if name = "Richard Francis" then getEmail = "FrancisR@nhlbi.nih.gov" end if if name = "Xin Xu" then getEmail = "XuX@nhlbi.nih.gov" end if if name = "Ying-Jie Wu" then getEmail = "WuY@nhlbi.nih.gov" end if if name = "Kaari Linask" then getEmail = "LinaskK@nhlbi.nih.gov" end if if name = "Heidi Dudik" then getEmail = "DudikH@nhlbi.nih.gov" end if if name = "Jay Noor" then getEmail = "NoorJ@nhlbi.nih.gov" end if if name = "Charlene Schramm" then getEmail = "SchrammC@nhlbi.nih.gov" end if if name = "Jesus Vazquez" then getEmail = "vazquezj@nhlbi.nih.gov" end if if name = "Julie Rosenthal" then getEmail = "rosenthj@nhlbi.nih.gov" end if if name = "Vipul Mangal" then getEmail = "mangalv@nhlbi.nih.gov" end if if name = "Brett Siegfried" then getEmail = "siegfrieb@nhlbi.nih.gov" end if if name = "Chris Spurney" then getEmail = "SpurneyC@nhlbi.nih.gov" end if if name = "Ed Doe" then getEmail = "doee@nhlbi.nih.gov" end if if name = "Linda Leatherbury" then getEmail = "LeatherL@nhlbi.nih.gov" end if if name = "Sarah Clauss" then getEmail = "ClaussS@nhlbi.nih.gov" end if if name = "Susan Riley" then getEmail = "rileys@nhlbi.nih.gov" end if if name = "emailForm" then getEmail = "emailForm" end if end function %> [an error occurred while processing this directive] <% name = Request.QueryString("name") email = getEmail(name) if Not IsEmailValid(email) then response.write("Send E-Mail to: ") response.write(name + " Valid e-mail address not found.
") else response.write("Send E-Mail to: ") response.write(name + "
") ''response.write(email + "
") validSendEmailFound = true end if %> High Throughput Mouse Genotyping Using Robotics Automation

High Throughput Mouse Genotyping Using Robotics Automation

File Downloads:

Automation Protocol- A detailed step-by-step description of the mouse-tail genotyping protocol (Download Automation Protocol as a Microsoft Word file).

AutomationScripts.zip - Archive of the FileMaker Pro 6 sample tracking database used to generate the .csv files for the MousePCR.MPT program on the Multiprobe II HT and LabChip software sample importing (requires FileMaker Pro 6…might not work correctly with conversion to FileMaker Pro 7 due to fundamental changes to file structures in the new version).

Mouse PCR- Multiprobe II HT program that combines template and master mix in a 96-well format for PCR given a .csv with sample details (requires Multiprobe WinPrep software).

Genotyping Automation – PowerPoint overview of the mouse genotyping automation protocol including examples of results.

For further information about the automation protocol please contact Kaari Linask or Dr. Cecilia Lo

<% end if %> <% if currentFolder <> "emailForm" and currentFolder <> "LCE Staff" then 'response.write "dummy statement" %> High Throughput Mouse Genotyping Using Robotics Automation

High Throughput Mouse Genotyping Using Robotics Automation

File Downloads:

Automation Protocol- A detailed step-by-step description of the mouse-tail genotyping protocol (Download Automation Protocol as a Microsoft Word file).

AutomationScripts.zip - Archive of the FileMaker Pro 6 sample tracking database used to generate the .csv files for the MousePCR.MPT program on the Multiprobe II HT and LabChip software sample importing (requires FileMaker Pro 6…might not work correctly with conversion to FileMaker Pro 7 due to fundamental changes to file structures in the new version).

Mouse PCR- Multiprobe II HT program that combines template and master mix in a 96-well format for PCR given a .csv with sample details (requires Multiprobe WinPrep software).

Genotyping Automation – PowerPoint overview of the mouse genotyping automation protocol including examples of results.

For further information about the automation protocol please contact Kaari Linask or Dr. Cecilia Lo

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Protocols

Genotyping Automations

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